in4mer Screen Size Calculator

Estimate cell numbers, gDNA requirements, and sequencing depth for your in4mer CRISPR/enAsCas12a pooled screen.

Quick Presets

Library Composition

Target genes

Paralog combinations Pairs, triples, quads (set 0 for single-gene only)

Guides per gene / combo

Non-targeting controls Recommended: 2-5% of library size

Screen Parameters

Coverage per construct

Biological replicates

Conditions / timepoints

MOI (multiplicity of infection)

Organism

Sequencing reads per construct

Screen Requirements